PhanerochaeteV1, Main, Exploration, bibRecord, 000B84

Cloning of Phanerochaete chrysosporium leu2 by complementation of bacterial auxotrophs and transformation of fungal auxotrophs.

Identifieur interne : 000B84 ( Main/Exploration ); précédent : 000B83; suivant : 000B85

Cloning of Phanerochaete chrysosporium leu2 by complementation of bacterial auxotrophs and transformation of fungal auxotrophs.

Auteurs : L S Zapanta [États-Unis] ; T. Hattori ; M. Rzetskaya ; M. Tien

Source :

Applied and environmental microbiology [ 0099-2240 ] ; 1998.

RBID : pubmed:9647839

Descripteurs français

English descriptors

KwdEn :
- 3-Isopropylmalate Dehydrogenase (MeSH), Alcohol Oxidoreductases (genetics), Amino Acid Sequence (MeSH), Base Sequence (MeSH), Basidiomycota (genetics), Fungal Proteins (genetics), Genetic Vectors (genetics), Molecular Sequence Data (MeSH), Sequence Alignment (MeSH), Sequence Homology, Amino Acid (MeSH), Transformation, Genetic (MeSH).
MESH :
- chemical , genetics : Alcohol Oxidoreductases, Fungal Proteins.
- chemical : 3-Isopropylmalate Dehydrogenase.
- genetics : Basidiomycota, Genetic Vectors.
- Amino Acid Sequence, Base Sequence, Molecular Sequence Data, Sequence Alignment, Sequence Homology, Amino Acid, Transformation, Genetic.

Abstract

A Phanerochaete chrysosporium cDNA library was constructed in an expression vector that allows expression in both Escherichia coli and Saccharomyces cerevisiae. This expression vector, lambda YES, contains the lacZ promoter for expression in E. coli and the GAL1 promoter for expression in yeast. A number of genes were cloned by complementation of bacterial amino acid auxotrophs. The cDNA encoding the beta-isopropylmalate dehydrogenase from P. chrysosporium was characterized further. The genomic clone (gleu2) was subsequently isolated and was used successfully as a selectable marker to transform P. chrysosporium auxotrophs for LEU2. Protoplasts for transformation were prepared with readily obtained conidiospores rather than with basidiospores, which were used in previous P. chrysosporium transformation procedures. The method described here allows other genes to be isolated from P. chrysosporium for use as selectable markers.

DOI: 10.1128/AEM.64.7.2624-2629.1998
PubMed: 9647839
PubMed Central: PMC106435

Affiliations:

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Le document en format XML

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<term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Basidiomycota (genetics)</term>
<term>Fungal Proteins (genetics)</term>
<term>Genetic Vectors (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
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<term>Transformation, Genetic (MeSH)</term>
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<term>Alcohol oxidoreductases (génétique)</term>
<term>Alignement de séquences (MeSH)</term>
<term>Basidiomycota (génétique)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Protéines fongiques (génétique)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Transformation génétique (MeSH)</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>3-Isopropylmalate Dehydrogenase</term>
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<term>Basidiomycota</term>
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<term>Vecteurs génétiques</term>
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<front><div type="abstract" xml:lang="en">A Phanerochaete chrysosporium cDNA library was constructed in an expression vector that allows expression in both Escherichia coli and Saccharomyces cerevisiae. This expression vector, lambda YES, contains the lacZ promoter for expression in E. coli and the GAL1 promoter for expression in yeast. A number of genes were cloned by complementation of bacterial amino acid auxotrophs. The cDNA encoding the beta-isopropylmalate dehydrogenase from P. chrysosporium was characterized further. The genomic clone (gleu2) was subsequently isolated and was used successfully as a selectable marker to transform P. chrysosporium auxotrophs for LEU2. Protoplasts for transformation were prepared with readily obtained conidiospores rather than with basidiospores, which were used in previous P. chrysosporium transformation procedures. The method described here allows other genes to be isolated from P. chrysosporium for use as selectable markers.</div>
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Cloning of Phanerochaete chrysosporium leu2 by complementation of bacterial auxotrophs and transformation of fungal auxotrophs.

Cloning of Phanerochaete chrysosporium leu2 by complementation of bacterial auxotrophs and transformation of fungal auxotrophs.

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Abstract

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